Ultrastructural alterations of osteocyte morphology via loaded implants in rabbit tibiae
Abstract
Osteocytes are crucial cells that control bone responses to mechanical loading. However, the effects of mechanical loading on osteocytes around dental implants are unclear. The aim of this study was to investigate whether mechanical loading via bone-integrated implants influences osteocyte number and morphology in the surrounding bone. Fourteen anodized Ti-6Al-4V alloy dental implants were placed in seven Japanese white rabbits, and implants in each rabbit were subjected to mechanical loading (50N, 3Hz for 1800 cycles, 2 days/week) along the implant long axis. Eight weeks after the initiation of loading, histomorphometric analysis and microcomputed tomography were performed. Scanning electron microscopy (SEM) was also performed with an acid etching technique using longitudinal and cross-sectional specimens. More bone formation around loaded implants was noted. In the implant neck, osteocytes tended to be more spherical with increased dendrite processes around loaded implants, while spindle-shaped osteocytes without increased dendrite processes were observed around unloaded implants in both longitudinal and cross-sectional images. In the bottom area, morphological changes in osteocytes were observed around loaded implants; however, dendrite processes did not differ in longitudinal or cross-sectional images, regardless of mechanical loading. These findings indicate that increased osteocyte numbers and developed dendrite processes are associated with anabolic bone responses to…
Read the paper
Related studies
- Irisin Enhances Osteoblast DifferentiationIn Vitro
- Glucocorticoids suppress Wnt16 expression in osteoblasts in vitro and in vivo
- Interaction between Schwann Cells and Osteoblasts In Vitro
- Administration of cinnamaldehyde promotes osteogenesis in ovariectomized rats and differentiation of osteoblast in vitro
- The Effect of the Major Components of Fructus Cnidii on Osteoblasts In Vitro
- Estrogen depletion alters osteogenic differentiation and matrix production by osteoblasts in vitro
Go deeper in Evidence Hub
Evidence Hub adds a study data sheet (design, sample size, evidence level, key numbers), an AI summary and a chat grounded in the paper's text.