SARM Rad140 Increases Osteoblasts, Muscle Fiber Size, Myonuclei, and Reduces Osteoclasts in Orchidectomized Wistar Rats
Abstract
Background: Orchidectomy is a surgical androgen deprivation therapy (ADT) for prostate cancer patients to achieve castrate testosterone levels. Selective androgen receptor modulators (SARMs) are used to mitigate the adverse effects of ADT, including elevated risk of osteoporosis, and reduced skeletal muscle mass. Rad140 is a novel SARMs that has high affinity for the androgen receptors. This study was conducted to determine the effects of SARM Rad140 on the number of osteoblasts, osteoclasts, muscle fiber cross-sectional area (CSA), muscle size, and number of myonuclei in rats underwent orchidectomy.Materials and Methods: An experimental study was conducted using a randomized post-test only control group design. Following orchidectomy, SARM Rad140 was administered orally for six weeks at various doses. Osteoblasts, osteoclasts, muscle fiber CSA, muscle size, and number of myonuclei were measured. Quantitative analysis was performed using one-way ANOVA.Results: There were significant differences in the effects of SARM Rad140 at different doses on osteoblast and osteoclast cells. At higher doses, the osteoblast cell counts in rats tended to increase, while the osteoclast counts tended to decline. The treatment group also showed significant results in the CSA of the gastrocnemius muscle fibers, as well as in the number of myonuclei of the gastrocnemius muscle.Conclusion: SARM Rad140 significantly increased the number of osteoblasts, muscle fiber CSA, and gastrocnemius muscle…
Read the paper
Related studies
- Irisin Enhances Osteoblast DifferentiationIn Vitro
- Glucocorticoids suppress Wnt16 expression in osteoblasts in vitro and in vivo
- Interaction between Schwann Cells and Osteoblasts In Vitro
- Administration of cinnamaldehyde promotes osteogenesis in ovariectomized rats and differentiation of osteoblast in vitro
- The Effect of the Major Components of Fructus Cnidii on Osteoblasts In Vitro
- Estrogen depletion alters osteogenic differentiation and matrix production by osteoblasts in vitro
Go deeper in Evidence Hub
Evidence Hub adds a study data sheet (design, sample size, evidence level, key numbers), an AI summary and a chat grounded in the paper's text.