Vitro Culture and Regulation Of Osteoblasts and Cellular Immunity Function In Multiple Myeloma patients
Abstract
Abstract Objective To investigate the biological characteristics of osteoblasts（OBs) cultured in vitro from bone marrow（BM) of multiple myeloma(MM) patients and to explore their generation and osteogenic potential. Then the affects by some factors such as bortezomib and MM patient serum on the OBs were observed. To explore the cellular immunity changes and the relationship between OBs and the immunity in MM. Methods OBs from BM of MM patients were cultured in vitro. The generation and osteogenic potential of OBs from MM patients and normal subjects were compared. The changes of their osteogenic potential and biological characteristics were observed. The antigens (CD34、CD138、CD45) on OBs and the quantities of T cell subsets，Dendritic cells(DC)，effective T cells、regulatory T cells and helper T cells (Th）were examined with flow cytometry assay. And then correlation was analyzed between OBs and the immunity. The levels of IL-7 were measured with ELISA. The BMP2 mRNAs were measured by RT-PCR. Results OBs from BM of MM patients could be cultured in vitro. The quantity of OBs from MM patients was less than normal subjects. There were calcium depositions in both groups after cultured for 4weeks culture. But the depositions of MM patients was less than that of normal controls. The OBs cultured with MM patient serum were less than those without patient serum. Bortezomib increased those from MM patients. CD138, CD45, CD34 were not detected on the cultured cells. The ratio of…
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