Generation of transgenic mice with osteoblast specific expression of h1-calponin
Abstract
Objective This study reported the establishment of a transgenic mouse strain with osteoblast specific expression of h1-calponin.Methods h1-calponin cDNA driven by collagen I promoter was constructed and introduced into the mouse by nuclear microinjection,and offsprings were produced.DNA was isolated from the tails of the mouse pups,and was used to detect the incorporation of h1-calponin mini gene by PCR using h1-calponin specific primers.The h1-calponin expression in osteoblast of transgenic mice was determined at mRNA level by RT-PCR.Results The PCR showed that there were one G0 transgenic mice with positive signal.RT-PCR showed h1-calponin was expressed in the osteoblasts of F1 transgenic mice.Conclusion h1-calponin cDNA has been incorporated into the offspring mice.Meanwhile a significant expression of h1-calponin mRNA was detected in the osteoblasts of F1 transgenic mice which offered an useful animal model to study the biological function of h1-calponin in skeleton development and diseases.
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