Polydopamine-assisted lithium ion loading on titanium alloy synergistically enhances osteogenesis and immunomodulation for bone regeneration: an In Vitro study
Abstract
BACKGROUND: Periodontal bone defects present significant challenges in clinical regeneration. Titanium mesh has been widely used in guided bone regeneration (GBR) due to its excellent mechanical properties, but its bioinert nature and potential immune response limit its regenerative efficacy. Surface modification of titanium alloys represents a promising approach to enhance their bioactivity. This study explores a novel strategy using polydopamine (PDA)-mediated lithium (Li) ion (Li+)loading on titanium surfaces to simultaneously promote osteogenesis and immunomodulation. METHODS: Titanium discs were modified through alkali-thermal treatment, PDA coating, and lithium chloride(LiCl) immersion at different concentrations (1, 5, and 10 mol/L). Surface characterization was performed using scanning electron microscopy (SEM) and X-ray photoelectron spectroscopy (XPS). Li+ release kinetics and surface hydrophilicity were systematically evaluated. In vitro biological effects were assessed using rat bone marrow stromal cells (rBMSCs) and RAW264.7 macrophages through comprehensive analyses of cell adhesion, proliferation, osteogenic differentiation (Alkaline phosphatase (ALP) activity, mineralization, and gene expression), and macrophage polarization (inflammatory cytokine profiling). Conditioned medium(CM) collected from the co‑culture of macrophages and Ti‑PDA‑5Li was further used to evaluate the effects of the immune microenvironment on the proliferation and osteogenic activity…
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