CFD-aided design and hydrodynamic characterization of a single-use perfusion bioreactor for high density cell culture
Abstract
To address the challenges of high-density animal cell culture, this study developed a high-density culture system comprising a single-use bioreactor (SUB) with a nominal volume of 500 mL and a pulsed tangential flow filtration (ITF) unit. The reactor can be configured with two layers of 35 mm diameter impellers-either double Elephant Ear (EE-EE) or Elephant Ear combined with Ribbon (EE-RB). The flow field characteristics were rigorously characterized through CFD simulations (60-240 rpm; 90-480 mL) validated by experimental data. Engineering analysis revealed the system's robust culture environment: [Formula: see text] values ranging from 2 to 15 h-1 (60-180 rpm; 200-400 mL; 30-150 mL/min aeration), [Formula: see text] values from 0.1 to 10 W/m3, and mixing times between 1 and 10 s. Crucially, the system maintains a mild shear environment with an average shear strain rate (SSR) below 25 s-1, fully within the physiological tolerance range for mammalian cells. This low-shear, high-mass-transfer design was validated through perfusion cultures of CHO and HEK293 cells. The system achieved exceptionally high cell densities of 8.32 × 107 cells/mL and 1.17 × 108 cells/mL, respectively, with cell viability consistently exceeding 90%, demonstrating its suitability for high-intensity biopharmaceutical production.
How strong is the evidence?
Evidence Hub reads this as a simulation / modelling, done in cell cultures (in vitro), not in people. Its evidence level is 5 of 5 (Very low · case / narrative / animal / in vitro / in silico). Case reports, narrative reviews, and animal, cell or computer-model studies give the weakest evidence for human health.
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