Effects of premarin on rat osteoblasts in vitro
Abstract
Objective To study the effect and mechanism of premarin on rat osteoblasts in vitro. Methods The osteoblasts were separated from the skull of new-born rats, and were cultured in 10% bovine serum DMEM. High and low dosage premarin was separately added and compared with blank group. In the end, osteoblasts were tested through MTT method. Expression of PKC and Bag-1 were assayed by Western-blot. Results Premarin promoted the multiplication of osteoblats cultured for 24 hours while the effect was even strengthened after 48 hours. Expression quantity of PKC and anti-apoptosis protein Bag-1 was increased remarkably. Conclusions Premarin prevents and treats osteoporosis by promoting multiplication of osteoblasts and enhancing the expression of PKC and anti-apoptosis protein Bag-1.
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