The effect of Nimodipine on culture of osteoblasts in vitro
Abstract
Objective To observe the effect of nimodipine on culture of osteoblasts in vitro Methods The culture medium with different nimodipine concentrations(10 -1 ～10 -9 g/L)and the second generation osteoblats(OB 2)from the skull of new born SD rats were mixed for the observation respectively about the proliferation(OD value at wavelength 570nm),the differentiation(ALP activity)and the mineralization(mineralized nodes/field of vision)of OB 2.Results The proliferation function was 0 12±0 01～0 41±0 04 OD value(mean and standard deviation);the ALP activity(mean and standard deviation)was 0 09±0 01U/mg protein and the mineralized nodes(mean and standard deviation)was 1 3±0 9.Conclusion In comparison with control groups,the proliferation and differentiation of the OB 2 were remarkably increased by 10 -6 g/L nimodipine concentration and the mineralization was significantly inhibited.The proliferation of the OB 2 was significantly inhibited with the increase of nimodopine concentration to 10 -2 g/L,the proliferation was of no effect when the concentration of nimodipine was decreased to 10 -8 g/L.
Read the paper
Related studies
- Irisin Enhances Osteoblast DifferentiationIn Vitro
- Glucocorticoids suppress Wnt16 expression in osteoblasts in vitro and in vivo
- Interaction between Schwann Cells and Osteoblasts In Vitro
- Administration of cinnamaldehyde promotes osteogenesis in ovariectomized rats and differentiation of osteoblast in vitro
- The Effect of the Major Components of Fructus Cnidii on Osteoblasts In Vitro
- Estrogen depletion alters osteogenic differentiation and matrix production by osteoblasts in vitro
Go deeper in Evidence Hub
Evidence Hub adds a study data sheet (design, sample size, evidence level, key numbers), an AI summary and a chat grounded in the paper's text.