Effect of icariine on the apoptosis of osteoblast in vitro
Abstract
Objective To observe the effect of icariine on apoptosis of osteoblasts in vitro and to study the possible mechanism of incariine on preventing and treating osteoporosis.Methods Calvarial osteoblasts were obtained by enzyme dissociation and mechanical isolation method from newborn SD rats,then osteoblasts of the fourth generation were divided into 5 groups randomly: 17 β-estradiol(1×10-8 mmol/l)group,low concentration of icariine group(1 ng/ml),middle concentration of icariine group(10 ng/ml),high concentration of icariine group(100 ng/ml) and control group.Different concentration of icariine were added into the culture mediums and apoptosis of osteoblasts were detected by TUNEL immunohistological staining technique and electron microscope after 48 hours.Results Apoptosis indices(AI) in different concentration icariine groups were decreased as compared with that of the control group(P0.05),while AI in the middle concentration icariine group(10 ng/ml) had the greatest difference(P0.01).There were less apoptotic osteoblasts in different icarrine groups than those in control group as studied by electron microscope scanning,especially in the middle concentration icariine group(10 ng/ml).Conclusion Icariine can suppress apoptosis of osteoblasts to promote bone formation and decrease bone absorption.This may be one of the possible mechanisms of incariine in treating osteoporosis.
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