Effect of Puerarin on Proliferation of Rat Osteoblast in Vitro
Abstract
Objective: To explore the effect of Puerarin on the proliferation of rat osteoblast in vitro.Methods: Newborn SD rat OBs were separated and cultured in vitro with the calvaria.The OBs of the third generation were cultured.The morphological characteristics of OBs were observed by inverted phase contrast microscope.Immunohistochemistry was used to examine the expression of typeⅠcollagen.Alkaline phosphatase(ALP) and calcifying nodule were stained.The osteoblasts cultured for 1,3,5 and 7 day were divided into experimental group at the culture medium doses ranging from 1×10-5 mol/mL to 1×10-10 mol/mL Puerarin and control group at the common culture medium without any Puerarin.The growth and proliferation ability was analyzed by CCK-8 method and growth curve was drawn.Results: For the OBs growth and proliferation ability by CCK-8 method,three days later,the cells grew fast obviously.The proliferation of cells ranging from 1×10-10 mol/L to 1×10-7 mol/L Puerarin was accelerated significantly after cultured for 5 and 7 days.Conclusion: Puerarin promotes proliferation of OBs and bone formation by the meditation of estrogen receptor.The osteoblasts of OBs treated doses ranging from 1×10-10 mol/L to 1×10-7 mol/L Puerarin was the most accelerated significantly.
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