Development-related regulation of plasma 1,25(OH)2D3 concentration by calcium intake in rat pups
Abstract
Circulating 1,25(OH)2D3 was determined in female rats between ages 1 and 20 weeks and in male rats between ages 5 and 12 weeks while the rats consumed diets containing vitamin D (5 U/g diet) and calcium concentrations of either 0.04, 0.4 or 1.6% Ca. When consuming the 0.4% Ca diet female rats had mean (+/- SE, pg/ml) serum 1,25(OH)2D3 values of 45 +/- 7 at 2 weeks, 272 +/- 23 at 4 weeks, 221 +/- 18 at 5 weeks and 51 +/- 6 and 49 +/- 4 at 13 and 20 weeks, respectively. Circulating 1,25 (OH)2D3 levels in the males at 4.5, 8 and 12 weeks were insignificantly higher than the levels for the females at comparable ages. There were no significant changes in plasma calcium over the ages examined in either sex. In order to test the efficiency of regulation of circulating 1,25(OH)2D3, 3-week-old female rats were given the 0.04% Ca diet for 2 or 6 weeks. At ages 5 and 9 weeks, serum 1,25(OH)2D3 values were 640 +/- 82 and 604 +/- 39 pg/ml, respectively, and therefore at least 3-fold higher than the values in rats consuming the 0.4% Ca diet. Plasma Ca concentrations in the calcium-deprived rats were 8.7 +/- 0.2 and 6.5 +/- 0.1 at 5 and 9 weeks, respectively. When the 0.04% Ca diet was introduced at 5 weeks of age, plasma Ca fell only 0.5 mg/dl and plasma 1,25(OH)2D3 rose to 1265 +/- 48 pg/ml. In contrast, plasma 1,25(OH)2D3 rose to only 176 +/- 19 pg/ml and plasma Ca fell 0.5 mg/dl when 12-week-old rats were given the 0.04% Ca diet for 5 weeks. When suckling rats were given access to the…
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