Determination of 17 beta-hydroxy-1 alpha-methyl-17 alpha-propyl-5-alpha-androstan-3-one in plasma by gas chromatography-mass spectrometry with single-ion detection
Abstract
The topical anti-androgen 17 beta-hydroxy-1 alpha-methyl-17 alpha-propyl-5 alpha-androstan-3-one is determined in plasma samples by extracting with ether and subsequent mass fragmentography with single-ion detection at m/z 303. 17 beta-Hydroxy-1 alpha-methyl-17 alpha-pentyl-5 alpha-androstan-3-one, added to the samples before extraction, is used as the internal standard. Reproducibility was calculated to be +/- 5.9% at the 5-ng/mL level and 0.4% at the 20-ng/mL level. The limit of detection is approximately 1 ng/mL. Total gas chromatography-mass spectrometry analysis time is approximately 10 min/sample.
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