59TH inner EAR biology workshop, 15–17 september 2024, warsaw, poland
Abstract
Introduction:The auditory nerve degenerates after severe damage to the organ of Corti including loss of hair cells and/or synapses.It is often assumed, based on the use it or lose it principle, that activation of the auditory nerve by chronic electrical stimulation (CES), as delivered by a cochlear implant (CI), halts further neural degeneration.However, data in both animal and human literature (e.g., Seyyedi et al., 2013) do not convincingly support that assumption.In the present study we examined the effect of CES on the auditory nerve in deafened guinea pigs, applying both structural and functional measurements.Material and methods: Normal-hearing guinea pigs received an intracochlear electrode array and were ototoxically deafened four weeks later by co-administration of kanamycin and furosemide.CES treatment started either one or five weeks after deafening, and it was applied for 6 days/week during 2 weeks.The CES stimuli consisted of asymmetric charge-balanced current pulses of 300 µA presented at quasi-random variable pulse rate (0.7-1.9 kHz; 1 kHz on average).Using a MED-EL PULSAR stimulator, awake eCAP recordings were weekly performed as described in Ramekers et al. (2022).Following the final eCAP recording session the animals were sacrificed, and their cochleas were processed for histological quantification of the spiral ganglion cells (SGCs).Results: SGC survival was similar for the implanted right and the non-implanted left ears in control animals.The SGCs in the…
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