Understanding local effects of orexin A and B on bone in vitro
Abstract
vivo, 3) to analyse impact of caspase inhibition on expression of autophagy related molecules in chondrocytes cultures in vitro.Based on temporospatial analysis, caspase-2 and caspase-8 are activated in non-apoptotic regions within the degrading part of the Meckel´s cartilage.Activation of pro-apoptotic caspases was demonstrated to occur also in non-apoptotic growth plate chondrocytes.The co-localization of these caspases with Beclin-1 suggests their possible involvement in the chondrocyte autophagic cell death.Moreover, caspase inhibition in limb derived chondrocytes caused a statistically significant impact on gene expression in the case of the autophagy related molecules and the engagement of individual caspases was uncovered.Thus the presented data open new perspective that are focused on the research into cartilage and bone development.This work was supported by the Grant Agency of the Czech Republic (19-12023S).
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