Biocatalysis of Immobilized Human Flavin-Containing Monooxygenase 1 on Nanostructured Surfaces
Abstract
Human flavin-containing monooxygenase 1 (FMO1) is a membrane protein bound to the endoplasmic reticulum expressed mainly in the kidney and involved in NADPH dependent oxidation reactions of soft nucleophilic heteroatoms of drugs, pesticides and dietary compounds.In this work, hFMO1 was heterologously expressed in E. coli and purified by affinity chromatography.The structure and functionality of purified protein were studied by using spectroscopical methods such as UV/Vis, CD, and Stopped-flow, differential scanning calorimetry and amperometry.The catalytic properties were tested in vitro and in vivo (in bacterial cells) using different substrates: drugs (methimazole, benzydamine, tamoxifen, dasatinib and vandetanib), pesticides (methiocarb, phorate and fenthion) and Contents Chapter 1 General Introduction ..........
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