Andrographolide Selectively Inhibits the Growth of LA7 Mammary Adenocarcinoma Cells
Abstract
High Resolution Image Download MS PowerPoint Slide Breast cancer (BC) causes significant mortality and morbidity, specifically in women globally. The first line of available therapies are far from satisfactory due to their adverse side effects, drug resistance, and reoccurrence of the cancer. As a result, natural source-based complementary medicines with anticancer activity and lesser side effects are widely investigated. Andrographolide (AGL), an active phytomolecule of Andrographis paniculata, exhibits diverse pharmacological activities against various diseases. The purpose of the study is to investigate the potential anticancer activity of AGL on LA7 cells, an in vitro mammary tumor model system, to understand its interaction with red blood cells (RBC) and effect on their membrane integrity. LA7 is a mammary adenocarcinoma cell line established by Renato Dulbecco in 1979 from the mammary tumor of Sprague–Dawley (SD) rat treated with 7,12-dimethylbenz[ a ]anthracene (DMBA). At first, a simple, rapid solvent extraction-gravimetric column chromatography method was employed for separation and purification of AGL from the dried leaf extract of A. paniculata . AGL showed significant anticancer activities on LA7 cells, with a potency similar to tamoxifen, an FDA-approved drug for BC. Mechanistic investigation showed that AGL triggers DNA damage, disruption of mitochondrial integrity, oxidative stress, cell cycle arrest at the G2/M phase, and apoptosis in LA7 mammary tumor cells.…
Read the paper
Related studies
- A Coupled SYSTEM of Intracellular Ca 2+ Clocks and Surface Membrane Voltage Clocks Controls the Timekeeping Mechanism of the Heart’s Pacemaker
- A Comprehensive Review on the Therapeutic Potential of Curcuma longa Linn. in Relation to its Major Active Constituent Curcumin
- Cancer epigenetics: Moving forward
- Current developments in LC-MS for pharmaceutical analysis
- Myricetin: A comprehensive review on its biological potentials
- A Computational Systems Biology Software Platform for Multiscale Modeling and Simulation: Integrating Whole-Body Physiology, Disease Biology, and Molecular Reaction Networks
Go deeper in Evidence Hub
Evidence Hub adds a study data sheet (design, sample size, evidence level, key numbers), an AI summary and a chat grounded in the paper's text.