New insights into understanding Xiongzhi Qufeng Zhitong Granule's anti-migraine effects and mechanism on reserpine-induced mice: from network pharmacology, molecular dynamics simulation, and experimental validation
Abstract
ETHNOPHARMACOLOGICAL RELEVANCE: Xiongzhi Qufeng Zhitong Granule (XZQF) is a classic traditional Chinese medicine (TCM) formula widely applied in clinical migraine treatment. Yet, the complex pathogenic mechanism of migraine remains incompletely clarified, which restricts the standardized clinical application and modern industrial transformation of XZQF. Therefore, further exploring its therapeutic effects and underlying molecular regulatory mechanisms against migraine is pivotal to support its clinical optimization and popularization.
AIM OF THE STUDY: This research aims to assessing the protective efficacy of XZQF against reserpine-triggered migraine injury in mice, with a core focus on exploring its modulatory effects on the 5-HT1B/TPH2/CGRP signaling axis, so as to supplement the mechanistic evidence for XZQF's anti-migraine effect.
MATERIALS AND METHODS: A reserpine-induced migraine mouse model was successfully established in this study. We then systematically evaluated the general behavioral observation, pain threshold detection, serum and tissue biochemical index quantification, and cerebral histopathological staining after XZQF intervention. Simultaneously, we integrated the multi-dimensional research strategies of network pharmacology, multiple molecular docking, and molecular dynamics (MD) simulation to unveil and verify the core candidate ingredients and therapeutic targets of XZQF used in treating migraine. Finally, immunohistochemistry (IHC), immunofluorescence…
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