Metformin-loaded J-AuPPS for infected diabetic wound treatment
Abstract
Introduction: Infected diabetic wounds pose a significant clinical challenge owing to the complex wound microenvironment. Various multifunctional synergistic therapeutic nanomaterials have been successfully developed to treat diabetic infected wounds.
Objective: A Janus Au-porphyrin polymersome heterostructure loaded with metformin (Met@J-AuPPS) is constructed and presented.
Methods: Porphyrin polymersome vesicles loaded with metformin (Met@PPS) was synthesized by supramolecular polymerization enhanced self-assembly. Metformin-loaded Janus gold-porphyrin polymersome (Met@J-AuPPS) was synthesized by photocatalytic synthesis method. Transmission electron microscope (TEM), scanning electron microscope (SEM), UV-vis absorption spectrum and ELISA experiment were used to detect the morphology, structure, photothermal properties and drug release properties of Met@J-AuPPS. The effects of Met@J-AuPPS on the proliferation and activity of HUVECs were detected by CCK-8 test, scratch test, Transwell test and tube formation test. The antibacterial and antibiofilm abilities of Met@J-AuPPS were detected by blood plate test, crystal violet staining, SEM of biofilm and bacterial live/dead staining. Imaging and histological staining were used to detect the efficacy of Met@J-AuPPS in treating diabetic wound infections in vivo.
Results: Excellent photothermal antibacterial performance against gram-positive methicillin-resistant Staphylococcus aureus (MRSA), gram-negative extended-spectrum…
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