LC–MS/MS metabolite profiling and in vivo antidiabetic, antilipidemic, and hepatoprotective potential of Ipomoea mombassana leaf extract with in silico validation
Abstract
Diabetes mellitus, often associated with dyslipidemia and hepatic dysfunction, requires effective plantderived therapeutics. This study evaluated the metabolite profile and pharmacological potential of the ethanolic
leaf extract of Ipomoea mombassana Vatke (IMLE). LC-MS/MS profiling identified 37 phytochemicals,
including flavonoids, alkaloids, terpenoids, and two previously unreported compounds, zinnimidine and
cinncassiol A 19-glucoside. Molecular docking demonstrated strong inhibitory activity of key metabolites against
α-amylase, with binding energies ranging from -7.6 to -9.8 kcal/mol. Tephrosin exhibited the most favorable
binding (-9.8 kcal/mol), stabilized by hydrogen bonds with HIS305 (74.9% occupancy) and TRP59 (52.2%).
Molecular dynamics simulations (100 ns) confirmed complex stability, with protein RMSD ~0.18 nm, ligand
RMSD 0.04-0.07 nm, and average MMGBSA free energy –50.73 kcal/mol. In vivo, IMLE (200 mg/kg)
significantly reduced fasting blood glucose from 344 ± 1.6 to 180 ± 3.9 mg/dL (≈48%; p<0.001), comparable to
metformin (175 ± 7.1 mg/dL), and improved oral glucose tolerance. Dyslipidemia was corrected, with total
cholesterol reduced by 38%, triglycerides by 35%, LDL-C by 68%, and HDL-C increased by 79% (all p<0.001).
Elevated SGOT and SGPT were reduced (71 → 42 U/L; 87 → 51 U/L; p<0.001). Antioxidant markers (SOD,
CAT, GSH) increased ~3-fold, while MDA decreased ~65% (p<0.001). Histopathology confirmed reversal of
pancreatic and hepatic damage. Overall,…
Read the paper
Related studies
- Metformin as an adjuvant in breast cancer treatment
- Metformin Lysosomal Targeting: A Novel Aspect to Be Investigated for Metformin Repurposing in Neurodegenerative Diseases?
- Aberrant HSF1 signaling activation underlies metformin amelioration of myocardial infarction in mice
- Metformin induces ZFP36 by mTORC1 inhibition in cervical cancer-derived cell lines
- Protein Interaction Network for Identifying Vascular Response of Metformin (Oral Antidiabetic)
- Preventing Long COVID With Metformin
Go deeper in Evidence Hub
Evidence Hub adds a study data sheet (design, sample size, evidence level, key numbers), an AI summary and a chat grounded in the paper's text.