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Metabolic changes in cancer cells after metformin treatment

Tia Tidwell, Abdelnour H. Alhourani

Figshare, 2021

Abstract

Proliferation measured by confluency in Incucyte ZOOM. Viability by CCK8, Alamar Blue, and direct cell counting microscopically (metformin concentration range: 1.5-24 mM). Metabolic analysis usuaing the Seahorse XF96 (metformin exposure for 48 hours, 3 mM). GLUT1 expression measured via flow cytometry. Detailed methods found in published article.

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