Nanochip-based precision medicine targeting cellular ageing
Abstract
Ageing is a major biological risk factor for many chronic diseases, and understanding its cellular mechanisms is important for developing targeted interventions for age-related issues. However, reliable biomarkers to quantify cellular changes in ageing remain limited. This study hypothesises that changes in nuclear envelope structure and intranuclear protein expression serve as holistic markers of ageing, and nanochip-based platforms enable their quantification by transforming irregular nuclear morphology into ordered deformation patterns. Furthermore, the efficacy of geroprotective compounds such as metformin and rapamycin can be evaluated through the restoration of consistent nanopillar-induced deformation patterns. Hence, this project aims to quantify cellular ageing across different life stages by probing changes in nuclear envelope structure using nanopillar-based platforms. Specifically, it aims to (i) establish a continuum of cellular life stages using TBHP as a controlled ageing model, and (ii) evaluate the effects of metformin and rapamycin on reversing ageing-associated changes in nuclear envelope integrity and intranuclear protein expression. A controlled in vitro model treated with graded concentrations of tert-butyl hydroperoxide (TBHP) to induce oxidative stress was employed to mimic a spectrum of cellular ageing states. Cells were subsequently treated with candidate anti-ageing compounds metformin and rapamycin, and nanochip-based platforms were employed to…
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