Whole-Transcriptome Profiling Reveals RNA Expression Dynamics and ceRNA Network Remodeling across Health, Sepsis, and Sepsis-Associated Acute Respiratory Distress Syndrome
Abstract
Abstract Background : Sepsis-associated acute respiratory distress syndrome (ARDS) is a severe manifestation of infection-related organ dysfunction characterized by dysregulated immune-inflammatory responses and tissue barrier disruption. However, whole-transcriptome alterations underlying the progression from sepsis to sepsis-associated ARDS remain insufficiently understood. This study aimed to characterize the expression patterns of mRNAs, long non-coding RNAs (lncRNAs), circular RNAs (circRNAs), and microRNAs (miRNAs) in peripheral blood from healthy controls, sepsis without ARDS, and sepsis-associated ARDS patients. Methods: 12 participants were enrolled, including 4 healthy controls (HC), 4 patients with sepsis without ARDS (SWA), and 4 patients with sepsis-associated ARDS (SA). Peripheral blood samples were subjected to whole-transcriptome sequencing. Trend analysis was performed to identify transcripts showing progressive increases or decreases across the HC–SWA–SA sequence, followed by Gene Ontology, Kyoto Encyclopedia of Genes and Genomes enrichment, target-gene prediction, and expression-correlation analyses to construct a competing endogenous RNA (ceRNA) network. Results: All sequencing datasets passed quality control. A total of 773 trend genes were identified, including 350 progressively upregulated and 423 progressively downregulated genes. These genes were mainly enriched in immune responses, cytokine production, extracellular matrix processes, MAPK and…
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