Long non-coding RNA and mRNA expression profiling of porcine satellite cells using strand-specific RNA-seq
Abstract
Skeletal muscle satellite cells are integral to muscle development and regeneration. Long non-coding RNAs (lncRNAs), a significant class of non-coding RNAs, participate in numerous biological processes and play critical roles in myogenic differentiation. However, research on the regulation of porcine satellite cell (PSC) proliferation and differentiation remains limited. To uncover key lncRNAs involved in PSC regulation, we performed strand-specific RNA-seq to comprehensively profile lncRNA and mRNA expression in PSCs at different stages of proliferation and differentiation (P24h, P48h, D18h, D28h). On average, 61.62 million clean reads were obtained across all samples. Through stringent filtering, we identified 1950 novel lncRNAs, 9367 annotated lncRNAs and 57252 mRNA transcripts for further analysis. This dataset provides valuable insight into the role of lncRNAs in PSC differentiation and contributes to a more detailed understanding of the molecular mechanisms underlying porcine muscle development.
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