Emergence of Aeromonas dhakensis‐linked motile Aeromonas septicemia in Pangasius hypophthalmus biofloc systems in Pakistan
Abstract
This study reports on an outbreak of motile Aeromonas septicemia (MAS) that caused significant mortalities in iridescent shark catfish (Pangasius hypophthalmus) in biofloc systems in the Punjab province of Pakistan. Clinical symptoms observed on the affected fish included anal fin hemorrhaging, mouth area bleeding, skin ulcerations, interstitial nephritis, and focal fusion of secondary lamellae. According to necropsy findings and a molecular phylogenetic analysis based on gyrB gene sequences, the etiologic agent of the disease was identified as Aeromonas dhakensis Ali.Pk01. The new isolate displayed resistance to multiple antibiotics such as ampicillin, cefuroxime sodium, meropenem, and penicillin, but remained susceptible to several antibiotics including amikacin, ceftazidime, chloramphenicol, ciprofloxacin, levofloxacin, and oxytetracycline. To fulfill Koch's postulates, fish were challenged by intraperitoneal injection with A. dhakensis Ali.Pk01, revealing its high virulence. Also, this isolate was found to encode multiple virulence genes including act, ela, alt, lip, and hyl. This study offers crucial information for local catfish farmers, highlighting the potential spread of virulent multidrug‐resistant pathogens and the need for better biosecurity and disease control measures without reliance on antibiotics.
Read the paper
Related studies
- Ferulic acid regulates the AKT/GSK-3β/CRMP-2 signaling pathway in a middle cerebral artery occlusion animal model
- Novel Pharmacokinetic/Pharmacodynamic Parameters Quantify the Exposure–Effect Relationship of Levofloxacin against Fluoroquinolone-Resistant Escherichia coli
- Photochemical behavior of levofloxacin
- Correlation Between Dissolution Profiles of Salt-Form Drugs in Biorelevant Bicarbonate Buffer and Oral Drug Absorption: Importance of Dose/ Fluid Volume Ratio
- Evaluation of the synergistic potential and mechanisms of action for de novo designed cationic antimicrobial peptides
- Synthesis of [11C]levofloxacin
Go deeper in Evidence Hub
Evidence Hub adds a study data sheet (design, sample size, evidence level, key numbers), an AI summary and a chat grounded in the paper's text.