Long non-coding RNA ANRIL promotes homologous recombination-mediated DNA repair by maintaining ATR protein stability to enhance cancer resistance
Abstract
Main text Aberrantly enhanced DNA damage repair leads to therapeutic resistance in many types of cancer [1, 2]. Recently, long non-coding RNAs (lncRNAs) have been shown as indispensable participants in DNA damage repair and provide novel targets for overcoming cancer resistance [3, 4]. However, the exact roles of most lncRNAs in DNA damage repair remain largely unknown. Here, we present our novel finding that lncRNA ANRIL promotes cancer resistance by mediating homologous recombination (HR) repair of DNA damage. ANRIL promoted HR repair to enhance cancer resistance to DNA damage treatments in lung cancer cells Lung cancer with high ANRIL expression is a leading cause of mortality worldwide and was chosen as a model to investigate the role of ANRIL in DNA damage repair and therapeutic resistance [5]. First, significant elevation of ANRIL was found in lung cancer tissues compared with that in adjacent normal tissues from 80 pairs of clinical specimens (Fig. S1A). High expression of ANRIL was also confirmed in lung cancer cells compared with that in normal BEAS-2B cells (Fig. S1B). Through analyzing data from TCGA database, ANRIL was also found to be upregulated in lung squamous cell carcinoma, but not adenocarcinoma (Fig. S1K). Moreover, ANRIL expression was greatly increased by ionizing radiation (IR) and other DNA damaging reagents, such as etoposide and camptothecin (CPT) (Fig. S1C-F), which was supported by the results of a previous study [6]. Then, genetically modified…
Read the paper
Related studies
- Regulation of nucleolus assembly by non-coding RNA polymerase II transcripts
- For a Healthy Histone Code, a Little SUMO in the Tail Keeps the Acetyl Away
- Discovering a binary CTCF code with a little help from BORIS
- Article Commentary: Stabilizing the Code–-Methods to Preserve RNA Prove Their Worth
- Foreword to the Special Issue for the RINTC (The Implicit Seismic Risk of Code-Conforming Structures) Project
- Commentary: Promoting Health and Well-being in Pediatric Primary Care Settings: Using Health and Behavior Codes at Routine Well-child Visits
Go deeper in Evidence Hub
Evidence Hub adds a study data sheet (design, sample size, evidence level, key numbers), an AI summary and a chat grounded in the paper's text.