Fitness effects of CRISPR/Cas9-targeting of long non-coding RNA genes
Abstract
1Department of Cellular & Molecular Pharmacology, University of California, San Francisco, San Francisco, CA, USA. 2Howard Hughes Medical Institute, University of California, San Francisco, San Francisco, CA, USA. 3California Institute for Quantitative Biomedical Research, University of California, San Francisco, San Francisco, CA, USA. 4Department of Neurological Surgery, University of California, San Francisco, CA, USA. 5Eli and Edythe Broad Center of Regeneration Medicine and Stem Cell Research, University of California, San Francisco, CA, USA. 6Center for Personal Dynamic Regulomes, Stanford University, Stanford, CA, USA. 7Department of Dermatology, Stanford University, Stanford, CA, USA. 8Department of Genetics, Stanford University, Stanford, CA, USA. 9Howard Hughes Medical Institute, Stanford University, Stanford, CA, USA. 10San Francisco Veterans Affairs Medical Center, San Francisco, CA, USA. 11Present address: Boston Children’s Hospital, Boston, MA, USA. ✉e-mail: [email protected] Long noncoding RNAs (lncRNAs) are a large class of transcripts that do not encode proteins, and while some lncRNAs are known to have important functions in mammalian cells, the function of the large majority of lncRNAs remains to be explored in depth. Thus, large-scale and unbiased approaches to screen for functional lncRNA loci are critical for the advancement of this field. Moreover, as any single approach will likely be affected by both false positives and false negatives,…
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