DIA-Based Plasma Proteomic Profiling Identifies Novel Biomarkers for Diagnosis of Premature Ejaculation and Their Association With Dapoxetine Treatment Outcomes
Abstract
BACKGROUND
Premature ejaculation (PE) is a highly prevalent male sexual dysfunction with undefined etiopathogenesis and a lack of validated objective biomarkers. Lifelong premature ejaculation (LPE) represents a more severe and persistent form of the disorder. Noninvasive plasma protein biomarkers for LPE diagnosis and their association with dapoxetine treatment outcomes remain insufficiently investigated.
OBJECTIVES
This study aims to identify and validate novel plasma protein biomarkers for LPE via data-independent acquisition (DIA) proteomics, construct a diagnosis model and clinical nomogram, and explore the association between these biomarkers and clinical outcomes after dapoxetine treatment.
METHODS
The study comprised a discovery phase and a validation phase from three centers. Initially, DIA proteomic analysis was performed on plasma samples from 5 LPE patients and 5 matched healthy controls. Differentially expressed proteins (DEPs) were identified and subsequently subjected to functional enrichment analyses, including GO, KEGG, and Reactome pathway analyses. Then, five core proteins were selected and validated using enzyme-linked immunosorbent assay (ELISA) in a larger cohort of 115 LPE patients and 60 healthy controls. LPE patients received 4-week on-demand dapoxetine treatment, with efficacy assessed by intravaginal ejaculatory latency time (IELT) and premature ejaculation diagnostic tool (PEDT) scores. The diagnostic model and the nomogram were constructed based…
How strong is the evidence?
Evidence Hub reads this as a cohort study, done in people. Its evidence level is 3 of 5 (Moderate · trial / cohort / secondary analysis). Trials without a large randomized design, cohorts and secondary analyses give moderate evidence. Reported size: 115 participants or records.
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