One-pot micellar augmented native fluorescence for facile fluorimetric assay of dapoxetine hydrochloride in biological plasma and tablets
Abstract
One of the medical problems is premature ejaculation which characterized by quick ejaculation and reaching orgasm rapidly. Dapoxetine was accepted as off-label antidepressant used in the treatment of premature ejaculation, so hereby we provide an innovative, non-extractive, environmentally safe protocol for the assay of dapoxetine in biological plasma and tablet. The principle of the assay is simple and only based on native fluorescence which was enhanced by micelle. Parameters influencing the method were optimized and measurements were accomplished at emission wavelength of 338 nm after excitation at 294 nm. The fluorescence-concentration plot was rectilinear over the range of 0.1-4 μg/mL. Directives of ICH guideline were the rules which followed to ensure the validity of the work. Eventually, the procedure was utilized in the dosage form assay and extended to include biological plasma analyses, with good percentage recuperation.
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