Interaction between phloretin and insulin: a spectroscopic study
Abstract
Abstract Diabetes is among the top ten deadly diseases in the world. It occurs either when the pancreas does not produce enough insulin (INS) or when the body cannot effectively use the insulin it produces. Phloretin (PHL) has a biological effect that can treat diabetes. A spectroscopic study was carried out to explore the interaction between phloretin and insulin. UV/Vis spectroscopy, fluorescence spectroscopy, and circular dichroism spectropolarimeter were used in the study. UV/Vis spectra showed that the interaction between PHL and INS produced strong absorption at a wavelength of 282 nm. The fluorescence analysis results showed that the excitation and emission occurred at 280-nm and 305-nm wavelengths, respectively. Temperature changes did not affect INS emissions. However, the interaction of PHL–INS caused a redshift at 305 to 317 nm. Temperature affected the binding constant ( K a ) and the binding site ( n ). Ka decreased with increasing temperature and increased the binding site. The thermodynamic parameters such as enthalpy (Δ H 0 ) and entropy (Δ S 0 ) each had a value of − 16,514 kJ/mol and 22.65 J/mol·K. PHL and INS interaction formed hydrogen bonds and hydrophobic interaction. The free energy (ΔG 0 ) recorded was negative. PHL and INS interactions took place spontaneously. The quenching effect was dynamic and static. K D values were greater than K S . The higher the temperature, the less was K D and K S . The appearance of two negative signals on circular…
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