Simultaneous Determination of Dapoxetine and 4 PDE5 Inhibitors in Aphrodisiac Health-Care Products by RP-HPLC
Abstract
A method for simultaneous determination of dapoxetine and 4PDE5inhibitors in aphrodisiac health-care products by RP-HPLC was proposed.The sample was dissolved in water and extracted with methanol. The extract(10μL)was introduced and separated by using Inertsil ODS-SP chromatographic column as stationary phase and mixtures of 0.8%(φ)triethylamine-0.4%(φ)acetic acid,acetonitile and 0.02%(φ)H3PO4solution with different mixing ratios as mobile phase in the gradient elution.UV-detection at 290nm was adopted in the determination.Linear relationships between values of peak area and mass concentration of tadalafil was kept in the range of 1.25-25.0mg·L-1 and for the other 4compounds in the same range of 5.0-100mg·L-1.Detection limits(3S/N)found were ranged from 0.05to 0.2mg·L-1.Values of recovery found by standard addition method were in the range of 94.1%-109%,with values of RSD′s(n=6)less than 1.0%.
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