Development and validation of HPLC/DAD method for simultaneously determination of six prohibited substances in model matrices
Abstract
The authorities have identified an emerging trend where over-the-counter products, represented as dietary supplements, contain hidden active ingredients that could be harmful. Consumers may unknowingly take products laced with varying quantities of approved prescription drug ingredients, controlled substances, and untested and unstudied pharmaceutically active ingredients. Hidden ingredients are increasingly becoming a problem in products promoted for sexual enhancement, weight loss, or bodybuilding. The tests have revealed the presence of some undesired substances like sildenafil, tadalafil, vardenafil, and their analogues in tainted sexual enhancement products. The content of these substances is usually around the daily curative dose. A simple high-performance liquid chromatography (HPLC) method for simultaneously determination of sildenafil, vardenafil, tadalafil, dapoxetine, yohimbine, and sibutramine was developed and validated. InfinityLab Poroshell 120 EC-C18 (150 '4.6 mm '4 μm particles) was used, as well as a diode-array detector (DAD) at 230 nm, and a gradient flow with 0.030 Eœ ammonium acetate buffer and acetonitrile. The method is linear in the following range: 2.5-37.5 μg/mL for yohimbine, 2.06-30.9 μg/mL for vardenafil, 2.0-30.0 μg/mL for sildenafil, 3.1-46.5 μg/mL for tadalafil, 1.98-29.7 μg/mL for dapoxetine, and 2.2-66.0 μg/mL for sibutramine. The linearity coefficient is R2 = 1 for all substances. Model matrices were spiked, and the analytical recoveries…
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