Determination of the Effect of Melatonin and Vitamin E on Cadmium Chloride-Induced Pathologies in the Stomach and Small Intestine of Lohmann Chicken Embryos

Erhan Şensoy, E. Güneş

Research and Practice in Veterinary and Animal Science, 2024

6 participants · 4 citations

Abstract

Cadmium (Cd), one of the most important heavy metals contributing to environmental

pollution, represents a significant risk factor for public health. The human body is

exposed to cadmium through the ingestion of food and respiration. Cadmium

accumulates in tissues such as the stomach and small intestine, where it can cause

pathological changes in the organs. Melatonin is an antioxidant hormone secreted by the

pineal gland, whereas vitamin E is a powerful antioxidant with therapeutic properties.

The aim of this study was to determine the toxicity of Cadmium chloride (CdCl2) in the

stomach and small intestine of Lohmann breed chicken embryos and to treat them with

melatonin and vitamin E.A total of 30 fertile eggs of the Lohmann breed were utilized

in this study, and five experimental groups were established (n=6): distilled water

(DW), DW+ (CdCl2), DW+Melatonin, DW+Vitamin E,

DW+CdCl2+Melatonin+Vitamin E. After a single dose of CdCl2 (0.430 mM),

Melatonin (5 mg) and vitamin E (3 mg) were administered to each egg via in-ovo

injection, the hole was closed with liquid paraffin. The fertile eggs were transferred to

the incubator and the study was completed before the chicks hatched (day 21). The

stomach and small intestine tissue of the chicks were excised, fixed in 10%

formaldehyde, and processed using an alcohol and xylene series. Following the

completion of routine histological procedures, the samples were stained with

Hematoxylin-Eosin and examined under a light microscope. A…

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