Phytochemicals Targeting Wnt/β-Catenin in Osteoblasts: Mechanisms, Scaffolds, and Computational Insights for Bone Therapeutics
Abstract
The Wnt/β-catenin signaling pathway is pivotal for osteoblast differentiation, bone formation, and skeletal homeostasis, with its dysregulation linked to osteoporosis and fracture non-union. Phytochemicals offer promising alternatives to unstable Wnt ligands, which risk tumorigenesis due to non-specific activation. In this study, we performed an extensive computational screening of selected phytochemicals-including quercetin, kaempferol, apigenin, genistein, gallic acid, ursolic acid, 2α-hydroxy ursolic acid, and berberine-previously reported to modulate the Wnt/β-catenin pathway. These compounds, derived from diverse phytochemical classes including flavonoids, isoflavones, phenolic acids, triterpenoids, and alkaloids, have been shown in prior experimental studies to promote osteogenic responses by stabilizing β-catenin, inhibiting GSK-3β, and upregulating osteogenic genes such as Axin2 and cyclin D1. To complement these findings, we employed computational approaches, including gene-drug interaction analysis (DGIdb), structural similarity screening (Data Warrior, > 90% similarity to known drugs), and molecular docking (Maestro Schrödinger Suite), to predict high-affinity interactions with key regulatory proteins in the Wnt pathway. Our docking results revealed high binding affinities of phytochemicals to key targets that are involved in the modulation of the Wnt/β-catenin pathway, such as Casein Kinase (CK, docking scores: -10.652 to -11.39), Estrogen Receptor (ER, docking…
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Evidence Hub reads this as a review, done in computer models (in silico), not in people. Its evidence level is 5 of 5 (Very low · case / narrative / animal / in vitro / in silico). Case reports, narrative reviews, and animal, cell or computer-model studies give the weakest evidence for human health.
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