Chestnut (Castanea sativa Mill.) leaf extract and pure castalagin reduce bacterial colonisation and the associated inflammation in gastric organoids and a mouse model of H. pylori infection
Abstract
Background: Helicobacter pylori is a bacterium that has been identified as a causative agent in the development of chronic gastritis, peptic ulcers, and gastric cancer. Current eradication therapies, based on antibiotic combination, are suffering from lack of patient compliance and primary antibiotic resistance. The traditional use and the scientific literature both report the gastroprotective role of natural extracts containing ellagitannins, suggesting an intriguing hypothesis about the potential impact on H. pylori-related gastritis. Anti-inflammatory and antibacterial effects of Castanea sativa Mill. extract standardized to contain castalagin have been previously demonstrated in human gastric epithelial cells.
Aim: This experimental work aimed at investigating the role of a food grade chestnut leaf extract in complex models of H. pylori infection.
Methods: The biological properties of chestnut leaf extract and castalagin have been validated, firstly in a novel murine gastric organoid cell model of H. pylori infection, and, secondly, in an in vivo infection model.
Results: Some NF-κB pathway genes, including ligands for CXCR2 and CXCR3 receptors, were found to be suppressed by both the extract and the pure compound. Furthermore, both strongly suppressed H. pylori colonisation and gastric inflammatory mediators in mice. The infiltration of immune cells, mainly neutrophils and monocytes, was also reduced.
Conclusion: The results of this work demonstrate the significant…
Read the paper
Related studies
- Ferulic acid regulates the AKT/GSK-3β/CRMP-2 signaling pathway in a middle cerebral artery occlusion animal model
- Novel Pharmacokinetic/Pharmacodynamic Parameters Quantify the Exposure–Effect Relationship of Levofloxacin against Fluoroquinolone-Resistant Escherichia coli
- Photochemical behavior of levofloxacin
- Correlation Between Dissolution Profiles of Salt-Form Drugs in Biorelevant Bicarbonate Buffer and Oral Drug Absorption: Importance of Dose/ Fluid Volume Ratio
- Evaluation of the synergistic potential and mechanisms of action for de novo designed cationic antimicrobial peptides
- Synthesis of [11C]levofloxacin
Go deeper in Evidence Hub
Evidence Hub adds a study data sheet (design, sample size, evidence level, key numbers), an AI summary and a chat grounded in the paper's text.