Colocasia esculenta corm Extract Attenuates Ethanol-induced Gastric Ulcer by Suppressing NF-κB Expression: An Integrated Network Pharmacology Approach and In Vivo Validation
Abstract
The corm of Colocasia esculenta (L.) Scott is widely used for food and treating gastric problems. However, its gastroprotective activity remains unexplored. This article aims to investigate the gastroprotective activity of the ethanol extract of C. esculenta corm (EECE) in ethanol-induced gastric ulcer in rats. EECE was analyzed for nutritional composition (proximate and vitamin C) and phytochemical composition (total phenol content, total flavonoid content, and quercetin levels), followed by metabolite profiling and an in vivo study. Male Wistar rats were randomly assigned to seven groups: (1) normal control, (2) negative control, (3) sucralfate group, (4) quercetin group, and three EECE groups at doses of (5) 200 mg/kg BW, (6) 400 mg/kg BW, and (7) 800 mg/kg BW. Following a two-week treatment period, all groups except the normal group were exposed to 70% ethanol. Post-mortem analysis included macroscopic and histopathological examination of gastric tissue, as well as Western blot analysis to investigate NF-κB p65 expression. EECE contains high moisture, ash, fat, and protein, and low carbohydrate levels, vitamin C at 417.73 mg/100 g, total phenol content of 833.43 mg GAE/100 g, total flavonoid content of 1,178.82 mg QE/100 g, and quercetin at 261.76 mg/100 g. UHPLC–HRMS/MS analysis revealed amino sugars, conjugated amino acids, lipids, phenolics, and minor cyanogenic glycosides. EECE significantly reduced ulcer area percentage in an ethanol-induced gastric ulcer model (p <…
How strong is the evidence?
Evidence Hub reads this as a preprint, done in animal and computer models (in silico), not in people. Its evidence level is 5 of 5 (Very low · case / narrative / animal / in vitro / in silico). Case reports, narrative reviews, and animal, cell or computer-model studies give the weakest evidence for human health.
Read the paper
Related studies
- Quercetin Influences Quorum Sensing in Food Borne Bacteria: In-Vitro and In-Silico Evidence
- Phenolic-protein interactions: insight from in-silico analyses – a review
- In Silico Discovery of Candidate Drugs against Covid-19
- Neuropharmacological Effects of Quercetin: A Literature-Based Review
- In vitro and in silico anti-dengue activity of compounds obtained from Psidium guajava through bioprospecting
- Effect of Quercetin Rich Onion Extracts on Bacterial Quorum Sensing
Go deeper in Evidence Hub
Evidence Hub adds a study data sheet (design, sample size, evidence level, key numbers), an AI summary and a chat grounded in the paper's text.