Antimicrobial Resistant Genes in Non-Typhoidal Salmonella Species in Food and Stool Samples in Port Harcourt, Rivers State, Nigeria
Abstract
Background: Non-typhoidal Salmonella (NTS) species are the major cause of foodborne infection.
Aim: To analyze antimicrobial-resistant genes found in non-typhoidal Salmonella species in food and stool samples in Port Harcourt using molecular methods.
Study Design: This was a cross-sectional study with simple randomized sampling.
Methodology: In this study, 12 Salmonella-positive isolates obtained from food and stool samples in Port Harcourt were subjected to antimicrobial sensitivity testing using the disk diffusion method of the commonly prescribed antibiotics in the city. Molecular techniques were employed to determine the presence of TET-W and CTX-M resistant genes, and also phylogenetic relationships.
Results: Out of 12 isolates [7 (3.3%) food samples and 5 (2.4%) stool samples], all (100.0%) were sensitive to ofloxacin, imipenem, levofloxacin, and gentamicin. High sensitivity was also observed to be ciprofloxacin (92.0%) and ceftriaxone (75.0%). However, complete resistance (100.0%) was recorded against tetracycline and cefotaxime. Resistance to cefpodoxime-proxetil (16.7%) and amikacin (8.3%) was exclusive to food isolates. Clinical isolates showed 20.0% resistance compared to 23.0% in food isolates, with none being sensitive to all antibiotics tested. Resistance patterns by antibiotic class indicated 0.0% resistance to fluoroquinolones, 4.2% to aminoglycosides, 25.0% to beta-lactams, and 100.0% to tetracycline. Multiple Antibiotic Resistance (MAR) analysis revealed…
How strong is the evidence?
Evidence Hub reads this as a randomized trial. Its evidence level is 2 of 5 (High · randomized trial). Randomized trials of a reasonable size are the strongest single-study design.
Read the paper
Related studies
- Ferulic acid regulates the AKT/GSK-3β/CRMP-2 signaling pathway in a middle cerebral artery occlusion animal model
- Novel Pharmacokinetic/Pharmacodynamic Parameters Quantify the Exposure–Effect Relationship of Levofloxacin against Fluoroquinolone-Resistant Escherichia coli
- Photochemical behavior of levofloxacin
- Correlation Between Dissolution Profiles of Salt-Form Drugs in Biorelevant Bicarbonate Buffer and Oral Drug Absorption: Importance of Dose/ Fluid Volume Ratio
- Evaluation of the synergistic potential and mechanisms of action for de novo designed cationic antimicrobial peptides
- Synthesis of [11C]levofloxacin
Go deeper in Evidence Hub
Evidence Hub adds a study data sheet (design, sample size, evidence level, key numbers), an AI summary and a chat grounded in the paper's text.