Anti-inflammatory effect of Quercetin and rapamycin in rat rheumatoid arthritis model by targeting PI3K/AKT/mTOR pathway
Abstract
Abstract Rheumatoid arthritis (RA) is one of the most prevalent chronic autoimmune disorders that primarily affects the articular tissue and is associated with significant systemic comorbidities. While numerous disease modifying anti-rheumatic drugs (DMARDs) are available for therapy, they are still not enough for most of patients to relieve pain. In addition to having several adverse effects, patients continue to have functional decline despite the use of these drugs. Quercetin, a natural bioflavonoid known for its potent anti-inflammatory and antioxidant properties, presents a promising alternative in the treatment of RA. Thus, our study estimated the effect of quercetin in a Freund’s Adjuvant-induced rat model of RA to test the effectiveness of quercetin through suppression of the PI3K/AKT/mTOR signalling pathways. Furthermore, we compared Quercetin's effects with those of Rapamycin (an established mTOR inhibitor) to validate the pathway's involvement in RA pathogenesis. Twenty-four rats Sprague Dawley of male sex were classified into four groups: negative control, arthritic group, rapamycin treated group, quercetin treated group. At the end of the study, before scarification, assessments of all the following were done, ankle diameter, arthritic score, and gaiting score. Then, animals were scarified, and the following markers were estimated: serum rheumatoid factor (RF) and CRP, and serum IL-1β and TNF-α. mTOR was estimated by western blotting and real-time PCR. p-AKT was…
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Evidence Hub reads this as a preprint, done in animal, not in people. Its evidence level is 5 of 5 (Very low · case / narrative / animal / in vitro / in silico). Case reports, narrative reviews, and animal, cell or computer-model studies give the weakest evidence for human health.
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