Quercetin alleviates painful diabetic neuropathy in association with gut microbial remodeling and spinal betaine-related metabolic changes
Abstract
BACKGROUND
Painful diabetic neuropathy (PDN) involves metabolic, inflammatory, and neuroimmune disturbances. Quercetin may alleviate diabetic neuropathy, but the associated gut-spinal mechanisms remain unclear.
PURPOSE
To investigate whether quercetin improves PDN in association with gut microbial remodeling, spinal betaine-related metabolism, and magnetic resonance imaging-derived spinal gadopentetate dimeglumine signal dynamics.
STUDY DESIGN
A two-stage study combined quercetin intervention, antibiotic-associated gut perturbation, multi-omics analyses, and independent betaine validation in a diet/streptozotocin-induced rat model of PDN.
METHODS
PDN rats received quercetin (30 or 60 mg/kg) or metformin. The 60 mg/kg dose was also evaluated during antibiotic co-treatment. Behavioral, metabolic, histological, inflammatory, oxidative-stress, immunofluorescence, western blot, fecal metagenomic, targeted spinal metabolomic, and magnetic resonance imaging assessments were performed. A separate cohort received intraperitoneal betaine.
RESULTS
Quercetin improved pain hypersensitivity, metabolic dysfunction, intestinal barrier-associated readouts, inflammation, spinal oxidative stress, magnetic resonance imaging-derived gadopentetate dimeglumine signal dynamics, and the overlap between aquaporin-4 and glial fibrillary acidic protein, with stronger overall effects at 60 mg/kg. Antibiotic co-treatment attenuated several quercetin-associated effects. Multi-omics analyses highlighted…
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