Detection of The Psla And Pela Genes in Multidrug-Resistant Pseudomonas Aeruginosa Isolated from Respiratory Infections
Abstract
Background: Pseudomonas aeruginosa is a leading opportunistic pathogen that have the ability to produce biofilms using secreted extracellular polymeric substances are typically controlled by the pslA and pelA genes. The current study was undertaken to determine the prevalence of pslA and pelA genes in MDR P. aeruginosa isolated from respiratory infections, and to assess its correlation with antibiotic resistance profile.
Methods: Cross-sectional study carried in Hilla Teaching Hospital, Babylon Province-Iraq during the period from July 2024 to January 2025. Methods: 87 patients who presented with clinically suspected respiratory tract infections. Antimicrobial susceptibility tests were conducted using the Kirby–Bauer disk diffusion method according to CLSI guidelines as follows: ceftazidime, imipenem, meropenem, ciprofloxacin, levofloxacin, gentamicin and amikacin. Molecular Detection of pslA and pelA GenesMultidrug-resistant isolates were selected to be used for the molecular detectionof the pslA genes by conventional PCR.
Results: Ciprofloxacin (85.0%) and levofloxacin (82.5%) had the highest resistance rates, followed by ceftazidime (77.5%), meropenem (75.0%), and imipenem (70.0%); however, amikacin had the lowest resistance rate at 45%. In molecular analysis, the pslA gene was identified in 95.0% of MDR isolates and pelA gene was found in 87.5%. The presence of pslA and pelA genes is significantly associated with resistance to β-lactams, carbapenems and fluoroquinolones…
How strong is the evidence?
Evidence Hub reads this as a cross-sectional. Its evidence level is 4 of 5 (Low · observational). Observational designs can show an association but not prove cause. Reported size: 87 participants or records.
Read the paper
Related studies
- Ferulic acid regulates the AKT/GSK-3β/CRMP-2 signaling pathway in a middle cerebral artery occlusion animal model
- Novel Pharmacokinetic/Pharmacodynamic Parameters Quantify the Exposure–Effect Relationship of Levofloxacin against Fluoroquinolone-Resistant Escherichia coli
- Photochemical behavior of levofloxacin
- Correlation Between Dissolution Profiles of Salt-Form Drugs in Biorelevant Bicarbonate Buffer and Oral Drug Absorption: Importance of Dose/ Fluid Volume Ratio
- Evaluation of the synergistic potential and mechanisms of action for de novo designed cationic antimicrobial peptides
- Synthesis of [11C]levofloxacin
Go deeper in Evidence Hub
Evidence Hub adds a study data sheet (design, sample size, evidence level, key numbers), an AI summary and a chat grounded in the paper's text.