Antimicrobial susceptibility and biofilm formation in clinical isolates of P. aeruginosa from patients at mosul hospital
Abstract
Objective: The study was designed to investigate the occurrence, distribution of samples, and antibiotic resistance and biofilm forming potential of P. aeruginosa. Method: This descriptive cross-sectional study, including 225 clinical samples, spanned from December 2018 to June 2019 and isolated P. aeruginosa were identified by traditional biochemical tests and confirmed by the VITEK 2 compact system. Antimicrobial susceptibility testing was done by Kirby-Bauer disk diffusion method according to CLSI 2025. Biofilm production was measured by Tissue Culture Plate method and Isolates were graded as weak, moderate and strong biofilm producers on the Basis of optical density. Results: A total of 12.8% (29/225) prevailed the recovery of P. aeruginosa and wound specimen displayed the maximum rate (22%, χ² = 18.55, p = 0.005). Antimicrobial susceptibility results showed that imipenem and levofloxacin were the most active (62.1% each), while gentamicin was found to be the least resisted (96.6%). Multidrug-resistant (MDR) and pandrug-resistant (PDR) patterns were found in 58.6% and 20.7% of the isolates. All isolates were shown to produce biofilm, with 17% weak, 48% moderate and 34% strong biofilm producers. Nevertheless, there was no significant correlation between the degree of biofilm formation and the profile of resistance to antimicrobials (p 0.05). Novelty: Although biofilm production was not directly associated with resistance profiles, its implication in chronic infections…
How strong is the evidence?
Evidence Hub reads this as a cross-sectional, done in people. Its evidence level is 4 of 5 (Low · observational). Observational designs can show an association but not prove cause.
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