The impact of lettuce-derived polyphenols on laying hens: Production performance, antioxidant status, immune function, and cecal microbiota profiles
Abstract
This study aimed to evaluate the effects of dietary supplementation with polyphenol-rich lettuce extract (PRLE) derived from the high-polyphenol lettuce cultivar Binfen-1 (BF-1) on production performance, antioxidant capacity, immune function, and cecal microbiota composition of Hyline Brown laying hens. A total of 140 healthy pre-laying hens (15 days before egg production) were randomly assigned to 4 groups: a control group fed a corn-soybean basal diet, and three experimental groups (TR1, TR2, TR3) fed the basal diet supplemented with 0.05%, 0.1%, and 0.15% PRLE, respectively. Diets were fed for 12 wk, afterwards 10 bird per group were euthanized for sampling blood and cecal digesta. Production performance monitoring showed that compared with the control group, 0.05% and 0.15% PRLE supplementation significantly increased the laying rate during Weeks 1-4 (p 0.05). For serum antioxidant indices, PRLE supplementation significantly reduced malondialdehyde (MDA) content and increased glutathione peroxidase (GSH-Px) activity across all experimental groups (p < 0.05). In terms of immunity, 0.1% PRLE significantly elevated serum immunoglobulin (Ig) G and IgM concentrations (p < 0.05). 16S rRNA gene sequencing revealed that PRLE supplementation increased the relative abundances of Firmicutes, Desulfobacterota, and Actinobacteriota while decreasing Proteobacteria at the phylum level(p < 0.05). At the genus level, it reduced the abundance of the conditional pathogen Peptococcus and…
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Evidence Hub reads this as a randomized trial, done in people. Its evidence level is 2 of 5 (High · randomized trial). Randomized trials of a reasonable size are the strongest single-study design.
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