Chonggu Granules attenuate cartilage injury in knee osteoarthritis patients with liver-kidney deficiency syndrome by regulating the Nrf2 pathway
Abstract
Objectives: To explore the mechanism mediating the ameliorative effect of Chonggu Granules on cartilage injury in knee osteoarthritis (KOA).
Methods: Bioinformatics approaches were used for analyzing the active ingredients of Chonggu Granules, their potential targets, the KOA- and ferroptosis-related genes, and their intersection genes. A compound-ingredient-disease-target network and the protein-protein interaction (PPI) network were constructed to identify the key active ingredients and core targets, which were verified by molecular docking studies. In the clinical trial, 62 KOA patients were randomly assigned into the observation group and control group (n=31) for treatment with glucosamine hydrochloride tablets and additional Chonggu Granules for 12 weeks, respectively, with 20 healthy participants serving as the healthy control group. Serum levels of cartilage injury markers (MMP-13, CTX-II, and COMP) and ferroptosis-related indices (GPX4, ACSL4, 4-HNE, and Fe2+) were measured using ELISA, and expressions of Keap1, Nrf2, SLC7A11, HO-1, and GPX4 mRNAs were determined with real-time PCR.
Results: Four key active ingredients (quercetin, eupatilin, kaempferol, and wogonin) of Chonggu Granules and 5 core targets (AKT1, TP53, IL-6, JUN, and Nrf2) were identified, which were involved in cancer, AGE-RAGE signaling, and the NFE2L2 (Nrf2) signaling pathways. Molecular docking showed strong binding between the active compounds and their targets, with NFE2L2 exhibiting the highest…
How strong is the evidence?
Evidence Hub reads this as a randomized trial, done in people. Its evidence level is 2 of 5 (High · randomized trial). Randomized trials of a reasonable size are the strongest single-study design. Reported size: 62 participants or records.
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