Phytochemical Analysis of Amorphophallus paeoniifolius (Dennst.) Nicolson and Its Standardisation by HPLC and HPTLC
Abstract
Elephant foot yams (Amorphophallus paeoniifolius (Denst.) Nicolson) are an important part of the diet in many parts of Asia. This study aims to analyse the physiochemical, phytochemicals, and in vitro antioxidant properties of different alcoholic and aqueous extracts. HPTLC and HPCL profile of ethanolic extract tubers of A. paeoniifolius was also investigated. Important secondary metabolites were detected through physicochemical analysis and phytochemical screening. In a laboratory, the antioxidant activity of A. paeoniifolius extracts was evaluated using standard free radical scavenging assays. HPLC testing showed that Gallic Acid, Quercetin, and Rutin could be used to standardise A. paeoniifolius. The study's results showed that it would give information that could be used to identify and verify this medicinal plant correctly and may help prevent its adulteration. The extracts of A. paeoniifolius have the potential to be a powerful antioxidant that can get rid of free radicals. The parameters for characterising A. paeoniifolius described in this paper could be used as a quality control standard for this plant.
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