Peripheral and central immune features in insomnia: Integrative bulk and single-cell transcriptomic analyses reveal core hub genes and candidate compounds
Abstract
Insomnia is closely associated with immune dysregulation, yet the overall pattern of peripheral-central immune disequilibrium and its underlying molecular basis remains incompletely understood. To characterize the peripheral-central immune features associated with insomnia, identify key immune cell populations and core molecular programs, and prioritize candidate therapeutic compounds with preliminary experimental validation. Peripheral blood bulk transcriptomic dataset GSE208668 was analyzed using differential expression analysis, weighted gene co-expression network analysis (WGCNA), and functional enrichment analysis to identify insomnia-associated genes. Protein-protein interaction network analysis and machine learning models were then applied in independent peripheral blood datasets to refine core genes. Immune deconvolution and peripheral blood single-cell transcriptomic dataset GSE213496 were used to determine the immune-cell context, cell-type localization, and intercellular communication features of these genes. The brain single-cell transcriptomic dataset GSE137665 was further analyzed to assess central alterations. Drug prediction, molecular docking, and molecular dynamics simulations were performed to prioritize candidate compounds, followed by in vitro validation of resveratrol in an LPS-induced THP-1 macrophage model. A total of 5,321 differentially expressed genes associated with insomnia were identified, and weighted gene co-expression network analysis…
Read the paper
Related studies
- Quercetin Influences Quorum Sensing in Food Borne Bacteria: In-Vitro and In-Silico Evidence
- Phenolic-protein interactions: insight from in-silico analyses – a review
- In Silico Discovery of Candidate Drugs against Covid-19
- Neuropharmacological Effects of Quercetin: A Literature-Based Review
- In vitro and in silico anti-dengue activity of compounds obtained from Psidium guajava through bioprospecting
- Effect of Quercetin Rich Onion Extracts on Bacterial Quorum Sensing
Go deeper in Evidence Hub
Evidence Hub adds a study data sheet (design, sample size, evidence level, key numbers), an AI summary and a chat grounded in the paper's text.