Anti-inflammatory effect of quercetin in a whole orbital tissue culture of Graves' orbitopathy
Abstract
PURPOSE: The authors previously reported that quercetin significantly inhibits interleukin (IL)-1β-induced increases of proinflammatory cytokines in cultured primary orbital fibroblasts from Graves' orbitopathy (GO). This study investigated the inhibitory effect of quercetin on inflammation in cultured whole orbital tissue.
METHODS: Orbital fat tissues from GO and normal samples were cultured with or without non-toxic concentrations of quercetin. Lactate dehydrogenase (LDH) release was used to identify non-toxic concentrations of quercetin. IL-6, IL-8, IL-1α, IL-1β and tumour necrosis factor alpha (TNFα) proteins were measured in tissue culture supernatants by ELISA, and gene transcript levels were determined using quantitative PCR, expressed as relative fold changes of threshold cycle value relative to the control group.
RESULTS: The maximal non-cytotoxic treatment of quercetin was 100 μM for 72 h, based on the considerably low LDH release with these conditions. IL-1β, IL-6 and TNFα protein levels corrected for tissue weight were significantly higher in supernatants of GO samples than normal controls (p<0.05). Quercetin reduced IL-6, IL-8 and TNFα protein production in supernatants of all GO samples (n=4) in a dose-dependent manner; however, only the reduction in IL-6 was statistically significant (p<0.05). Quercetin had a significant suppression of tissue IL-6, IL-8, IL-1β and TNFα mRNA expression in cultured orbital tissues from three GO samples relative to untreated…
Read the paper
Related studies
- Quercetin Influences Quorum Sensing in Food Borne Bacteria: In-Vitro and In-Silico Evidence
- Phenolic-protein interactions: insight from in-silico analyses – a review
- In Silico Discovery of Candidate Drugs against Covid-19
- Neuropharmacological Effects of Quercetin: A Literature-Based Review
- In vitro and in silico anti-dengue activity of compounds obtained from Psidium guajava through bioprospecting
- Effect of Quercetin Rich Onion Extracts on Bacterial Quorum Sensing
Go deeper in Evidence Hub
Evidence Hub adds a study data sheet (design, sample size, evidence level, key numbers), an AI summary and a chat grounded in the paper's text.