Bioavailability of quercetin for in vitro fibroblast culture
Abstract
The article is devoted to the assessment of cellular bioavailability of a flavonoid widely distributed in the plant kingdom - quercetin. Using high-performance liquid chromatography, the absorption of quercetin by human dermal fibroblasts in vitro was studied by the residual content in the culture fluid during incubation with different concentrations: 400, 200, 100, 15, 7.5, 5, 3, 1.5 and 1 μM. Therapeutic and prophylactic models were used to assess the content of quercetin in the culture fluid with its initial addition at concentrations of 50 and 150 μM and in the prophylactic model - 0.1 and 1 μM. The fibroblast culture absorbs quercetin in the range from 124 to 198 times. The qualitative composition of the culture fluids did not change. It was found that quercetin was detected in the culture liquid at a concentration of about 0.3 μM when 150 μM quercetin was added to the cells in the preventive and therapeutic models. In other cases, this flavonoid was not detected.
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