Biotransformation of quercetin by Bacillus subtilis and anticancer activity evaluation: in vitro and in Silico
Abstract
Biotransformation of quercetin by Bacillus subtilis ATCC 23,857 yielded a major metabolite; apigenin-7-O-pentoside (A7P) and 9 other phenolic metabolites. They were elucidated by LC-ESI-TOF-MS/MS analysis. Furthermore, the cytotoxic potential of the biotransformed products was compared to their parent substrate (S) using MTT assay against 2 cancer cell lines; A549 and Caco-2. After 72 h. incubation, the BPs showed concentration-dependent cytotoxic activity against Caco-2 and A549 with IC50 equal to 0.32 and 0.78 mg/ml, respectively. Both BPs and S were safe on the normal human skin fibroblast (hFB) cell line. Molecular docking confirmed the high binding affinity of A7P towards phosphatidyl inositide 3-kinase delta (PI3Kδ) and Cathepsin B protein (CatB) expressed in lung and colorectal cancer cells compared to quercetin suggesting higher anticancer activity. The present findings suggest that the application of biotransformation techniques using bacteria as B. subtilis can enhance the biological activity of flavonoids by generating bioactive metabolites.
Read the paper
Related studies
- Quercetin Influences Quorum Sensing in Food Borne Bacteria: In-Vitro and In-Silico Evidence
- Phenolic-protein interactions: insight from in-silico analyses – a review
- In Silico Discovery of Candidate Drugs against Covid-19
- Neuropharmacological Effects of Quercetin: A Literature-Based Review
- In vitro and in silico anti-dengue activity of compounds obtained from Psidium guajava through bioprospecting
- Effect of Quercetin Rich Onion Extracts on Bacterial Quorum Sensing
Go deeper in Evidence Hub
Evidence Hub adds a study data sheet (design, sample size, evidence level, key numbers), an AI summary and a chat grounded in the paper's text.