In-silico receptor interactions, phytochemical fingerprint and biological activities of Matricaria chamomilla flower extract and the main components
Abstract
In this study, total phenolic and flavonoid content, quantitative phenolic analysis, in-vivo, in-vitro and in-silico biological activities of Matricaria chamomilla flower extracts collected from Bulancak (Giresun) were investigated. Phenolic content was determined by LC–MS/MS and antioxidant, antibacterial, antifungal, antigenotoxic and anti-inflammatory activities of the extract and the main components were investigated. Caffeic acid, quercetin and kaempferol were detected as major compounds in the flower extract by LC–MS/MS analysis and the detected levels were 165.085 mg/kg, 112.673 mg/kg and 67.417 mg/kg, respectively. The DPPH radical scavenging activity of M. chamomilla flower extract ranged from 12.4 to 81.1%, while superoxide anion inhibition was observed between 10.6 and 65.8%. Even at low doses, the main components, caffeic acid, quercetin and kaempferol, alone show more potent antioxidant activity. Flower extract was effective against both gram-positive, gram-negative bacteria and fungi and exhibited a broad spectrum antimicrobial effect. Three main components showed a lower sidal effect than the flower extract. M. chamomilla flower extract showed significant antigenotoxicity by reducing NaN3-induced micronucleus formation by 58%, while the main components showed a lower activity. M. chamomilla flower extract, showed a protein denaturation inhibition in the range of 23.5% to 71% and its IC50 value was 408 µg/mL. The IC50 values of caffeic acid, quercetin and…
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