Enhanced Antibacterial Activity of Highly Biocompatible Polymeric Core-Shell Levofloxacin Gold Nanocomposite Formulation Against Pseudomonas aeruginosa
Abstract
Using natural and synthetic polymers as the components for the core-shell nanocomposite preparation has received recent attention in biomedicine due to their high biocompatibility, high efficacy, and biodegradability. In this present investigation, chitosan-polyvinyl alcohol core-shell gold nanocomposite was synthesised adopting green science principles followed by fabrication with fluoroquinolone antibiotic levofloxacin (LE-CS-PVA-AuNC). Core-shell nanocomposite was prepared from biogenic gold nanoparticles, chitosan, polyvinyl alcohol polymer mixture, and levofloxacin under optimum conditions, and the synthesised nanocomposite exhibited a highly stable nanoarchitecture. Enhancement of antibacterial activity of the nanocomposite was evaluated against the clinical strain of Pseudomonas aeruginosa by determination of growth inhibition, survival rate parameters, and biofilm inhibition rate. Levofloxacin-fabricated core-shell nanocomposite brought about higher growth inhibition than the free levofloxacin, which was confirmed by a notable zone of inhibition, growth inhibition at a lower concentration, rapid biofilm inhibitory rate, and changes in survival growth parameters. In vitro drug release pattern was studied by continuous dialysis, which reveals that the nanocomposite exhibited controlled, sustained release pattern and cumulative release reached almost 98.0% at 72 h. Biocompatibility was studied with human keratinocytes (HaCaT cell line), which was studied by measuring…
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Evidence Hub reads this as a experimental / empirical study, done in cell cultures (in vitro), not in people. Its evidence level is 5 of 5 (Very low · case / narrative / animal / in vitro / in silico). Case reports, narrative reviews, and animal, cell or computer-model studies give the weakest evidence for human health.
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