Covalently Cross-Linked Chitosan Hydrogel Sheet for Topical Ophthalmic Delivery of Levofloxacin
Abstract
In the present study, a covalently cross-linked chitosan hydrogel sheet was fabricated and assessed as an ophthalmic carrier for topical delivery of levofloxacin. Upon mixing 4-arm polyethylene glycol with aldehyde end groups (4-arm PEG-CHO) with glycol chitosan (GC) at various feed ratios, a series of hydrogel sheets formed spontaneously under mild conditions. Rheological characterization illustrated that the gelation time and moduli of the hydrogels could be tuned by controlling the component concentrations. The swelling ratio of hydrogels increased with decreasing 4-arm PEG-CHO and GC concentrations. The proposed hydrogel exhibited a typical porous structure, with pore size in the range 20-150 μm. This structure allowed the encapsulated levofloxacin to be released from the hydrogel matrix via diffusion within the first 0.5 h, followed by a sustained release up to 24 h. In vitro cytotoxicity and biocompatibility assays showed that the proposed hydrogels are relatively noncytotoxic and have excellent cytocompatibility with L-929 cells after 24 h of incubation. Furthermore, an ocular irritation test demonstrated the non-irritant character of the proposed hydrogel after topical application. These characteristics support the potential use of covalently cross-linked chitosan hydrogel sheets in topical ophthalmic drug delivery applications.
Read the paper
Related studies
- Ferulic acid regulates the AKT/GSK-3β/CRMP-2 signaling pathway in a middle cerebral artery occlusion animal model
- Novel Pharmacokinetic/Pharmacodynamic Parameters Quantify the Exposure–Effect Relationship of Levofloxacin against Fluoroquinolone-Resistant Escherichia coli
- Photochemical behavior of levofloxacin
- Correlation Between Dissolution Profiles of Salt-Form Drugs in Biorelevant Bicarbonate Buffer and Oral Drug Absorption: Importance of Dose/ Fluid Volume Ratio
- Evaluation of the synergistic potential and mechanisms of action for de novo designed cationic antimicrobial peptides
- Synthesis of [11C]levofloxacin
Go deeper in Evidence Hub
Evidence Hub adds a study data sheet (design, sample size, evidence level, key numbers), an AI summary and a chat grounded in the paper's text.